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Journal Club August 29th at 13:00 EST/18:00 UK

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The next August Journal Club will be on Tuesday August 29th at 13:00 EST/18:00 UK and will be streamed live from Cooper Union to our Facebook page.

We have been talking about a number of new studies in past Journal Clubs, so we thought it was time to take a look at the actual delivery methods and the hurdles we face in the field to develop effective therapies.

We will be discussing the delivery methods for gene therapies and in particular this recent paper:

Gallego-Perez, D., Pal, D., Ghatak, S., Malkoc, V., Higuita-Castro, N., Gnyawali, S., … & Singh, K. (2017). Topical tissue nano-transfection mediates non-viral stroma reprogramming and rescue. Nature nanotechnology.

CategoryJournal club
About the author
Oliver Medvedik

Oliver Medvedik

Oliver Medvedik, Co-founder of Genspace citizen science laboratory in Brooklyn NY, earned his Ph.D. at Harvard Medical School in the Biomedical and Biological Sciences program. As part of his doctoral work he has used single-celled budding yeast as a model system to map the genetic pathways that underlie the processes of aging in more complex organisms, such as humans. Prior to arriving in Boston for his doctoral studies, he has lived most of his life in New York City. He obtained his bachelor’s degree in biology from Hunter College, City University of New York. Since graduating from Harvard, he has worked as a biotechnology consultant, taught molecular biology to numerous undergraduates at Harvard University and mentored two of Harvard’s teams for the international genetically engineered machines competition (IGEM) held annually at M.I.T. Oliver is also the Director of The Maurice Kanbar Center for Biomedical Engineering at the Cooper Union, New York City. The Maurice Kanbar Center for Biomedical Engineering is open to all Cooper Union faculty and students working on bioengineering projects requiring equipment and space for tissue culture, genetic engineering, biomechanics, and related research. Faculty that is currently using the facility are pursuing groundbreaking biomedical research in such fields as biomedical devices, tissue engineering, obstructive sleep apnea biomechanics also collaborating with several major New York City-based hospitals. The Kanbar Center continues to provide space for undergraduate teams participating in the international genetically engineered competition (iGEM) during the summer, as well as space for courses that offer a biological laboratory component.
  1. James Joyce
    August 27, 2017

    It would be cool if there could be some discussion on megaTALs and whether or not they are the answer gene editing is looking for?

    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3936731/

    “MegaTALs exhibit dramatic increases in cleavage activity compared with their isolated mn cleavage heads, an effect that appears to be an outcome of the auxiliary TAL effector DNA-binding domain docking (‘addressing’) the mn adjacent to its target and effectively increasing the rate of enzyme–substrate complex formation. Furthermore, the mn-mediated cleavage produces DNA ends with 3′ overhangs that are efficiently processed by the Trex2 exonuclease, thus allowing the co-expression of a megaTAL and Trex2 to achieve unprecedentedly high targeted modification rates in primary cells of >70%.”

  2. James Joyce
    August 27, 2017

    Also could the recently developed technology of Spherical Nucleic Acids delivering whole enzymes to cells be useful?

    DNA-Mediated Cellular Delivery of Functional Enzymes:

    https://pubs.acs.org/doi/abs/10.1021/jacs.5b09711

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