Oocytes Age Slowly by Remodeling Electron Transport

A fundamental complex is deactivated until it's ready to be used.


Early embryoEarly embryo

Scientists have discovered that immature oocytes maintain their youth by shutting down one of the protein complexes that produce energy in mitochondria [1].

Live slow, stay young

Every female is endowed with a finite number of oocytes that form before birth, then reside in the ovaries in a dormant state until maturation and release. Oocytes are faced with a tricky task: they must remain in good shape throughout the body’s reproductive span in order to be able to produce healthy offspring. Interestingly, in addition to oocytes aging very slowly, early embryos undergo an enigmatic rejuvenation event so that the offspring produced by the adult organism starts from age zero [2].

However, as time passes, oocyte quality begins to dwindle, contributing to reproductive dysfunction, which occursΒ  earlier than many other manifestations of aging. Researchers have been trying to understand the mechanisms involved, but with limited success. In this new groundbreaking study published in Nature, scientists from Spain made a huge leap forward, showing that oocytes modify one of the most important cellular processes so that it produces less damage.

Less energy, less byproducts

Cells acquire energy mostly via the intricate, multi-stage process known as the electron transport chain (ETC), which uses nutrients that we get from food to produce adenosine triphosphate (ATP), the ‘energy currency’ of the cell. Sadly, the system is not perfect. As professor Vadim Gladyshev (who was not involved in this study) explained in his recent interview with Lifespan.io, all biological processes produce damage as a byproduct, and the indispensable ones can produce a lot of it.

The byproducts of the ETC are the infamous reactive oxygen species (ROS). While they also do some useful work such as cellular signaling, ROS mostly just wreak havoc, inducing DNA damage and disrupting various cellular processes [3]. Mitochondria are the main, though not the only, source of endogenous ROS.

Since ROS are linked to cellular aging [4], the researchers began with analyzing their levels in human and frog early-stage (immature) oocytes. Amazingly, neither produced any detectable ROS signal. The researchers then determined that the absence of ROS resulted not from their fast degradation by molecules called ROS scavengers, but from decreased ROS production. They also measured mitochondrial membrane potential which is higher when ETC is hard at work. In early-stage oocytes, membrane potential was remarkably low. Together with the low levels of mitochondrial ROS, this indicated reduced ETC activity.

Which one of the five?

The ETC is powered by five protein complexes (large molecules constructed from several different proteins), numbered from I to V. To pinpoint the particular complex that was responsible for the strange behavior of the ETC, the researchers tried shutting down the complexes one by one. Blocking any complex other than Complex I resulted in the oocytes’ death: in other words, only Complex I was not involved in any essential activity. Importantly, Complex I is also the foremost producer of ROS.

Proteomic analysis revealed that in early-stage oocytes, the levels of all ETC subunits (complex-forming proteins) were lower than in mature oocytes, with Complex I subunits almost completely absent. Somehow, oocytes managed to remodel their ETC to exclude Complex I! To date, in no other animal cell type that contains functioning mitochondria, absence of Complex I has ever been detected, and in the plant realm, the only known exception is mistletoe.

Oocytes apparently pay for this trick with diminished overall ETC activity, which is probably just enough to support immature oocytes in their dormant mode. As the researchers discovered, the ETC goes back on track – completely with Complex I – in mature oocytes that must do the heavy lifting of forming a new organism. Predictably, ROS levels also shot up in mature vs early-stage oocytes.

To sum things up, early-stage oocytes reside in a sort of hibernation: they are not really doing anything other than simply surviving, but they also accumulate very little damage. As the researchers note, stem cells, particularly neuronal and hematopoietic stem cells, also exhibit low ETC activity and ROS levels [5], and they are known to age slowly. The next step would be to determine whether stem cells also shut down their Complex I.


This groundbreaking study uncovers a previously unknown mechanism by which oocytes, and maybe also stem cells, greatly reduce their rate of aging. As mature organisms, we need our cells to do stuff (while accumulating damage) rather than lie dormant, so this discovery, as fascinating as it is, might not be immediately translatable into anti-aging therapies. However, it can teach us a lot about aging in general and reproductive aging in particular and, hence, about possible ways to counter it.

We would like to ask you a small favor. We are a non-profit foundation, and unlike some other organizations, we have no shareholders and no products to sell you. We are committed to responsible journalism, free from commercial or political influence, that allows you to make informed decisions about your future health.

All our news and educational content is free for everyone to read, but it does mean that we rely on the help of people like you. Every contribution, no matter if it’s big or small, supports independent journalism and sustains our future. You can support us by making a donation or in other ways at no cost to you.

Creating a Noise Clock to Measure Biological Age

Publishing in Aging, the Conboy research lab has outlined the problems with existing machine learning-based clocks and created a new...

EARD2023: Using NFTs to Support Video Gaming for Good

In this talk, Lifespan.io President Keith Comito describes use cases for "Proof of Philanthropy" (PoP) dynamic NFTs - a new...

Senolytics as a Potential Back Pain Treatment

In a recent paper, researchers from McGill University in Canada have investigated how a combination of two senolytics, RG-7112 and...

A Potential Exercise Mimetic to Restore Youthful Memory

Researchers have identified a platelet-derived factor that improves cognition in mice and published their findings in Nature Communications. Finding the...


[1] RodrΓ­guez-Nuevo, A., Torres-Sanchez, A., Duran, J.M. et al. Oocytes maintain ROS-free mitochondrial metabolism by suppressing complex I. Nature (2022).

[2] Kerepesi, C., Zhang, B., Lee, S. G., Trapp, A., & Gladyshev, V. N. (2021). Epigenetic clocks reveal a rejuvenation event during embryogenesis followed by aging.Β Science Advances,Β 7(26), eabg6082.

[3] Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., … & Bitto, A. (2017). Oxidative stress: harms and benefits for human health.Β Oxidative medicine and cellular longevity,Β 2017.

[4] Shields, H. J., Traa, A., & Van Raamsdonk, J. M. (2021). Beneficial and detrimental effects of reactive oxygen species on lifespan: A comprehensive review of comparative and experimental studies.Β Frontiers in Cell and Developmental Biology,Β 9, 628157.

[5] Khacho, M., Harris, R., & Slack, R. S. (2019). Mitochondria as central regulators of neural stem cell fate and cognitive function.Β Nature Reviews Neuroscience,Β 20(1), 34-48.

About the author
Arkadi Mazin

Arkadi Mazin

Arkadi is a seasoned journalist and op-ed author with a passion for learning and exploration. His interests span from politics to science and philosophy. Having studied economics and international relations, he is particularly interested in the social aspects of longevity and life extension. He strongly believes that life extension is an achievable and noble goal that has yet to take its rightful place on the very top of our civilization’s agenda – a situation he is eager to change.
No Comments
Write a comment:


Your email address will not be published.

This site uses Akismet to reduce spam. Learn how your comment data is processed.