The Journal Club, hosted by Dr. Oliver Medvedik, returns to the Lifespan.io Facebook page at 12:00 on Tuesday, February 28th. This month, we are covering a recent paper that showed a method of restoring youthful collagen production in aged skin . If you would like some background, check out our article ‘New Way to Help Aging Cells Produce Collagen‘, which covers this paper.
The success of messenger RNA therapeutics largely depends on the availability of delivery systems that enable the safe, effective and stable translation of genetic material into functional proteins. Here we show that extracellular vesicles (EVs) produced via cellular nanoporation from human dermal fibroblasts, and encapsulating mRNA encoding for extracellular-matrix α1 type-I collagen (COL1A1) induced the formation of collagen-protein grafts and reduced wrinkle formation in the collagen-depleted dermal tissue of mice with photoaged skin. We also show that the intradermal delivery of the mRNA-loaded EVs via a microneedle array led to the prolonged and more uniform synthesis and replacement of collagen in the dermis of the animals. The intradermal delivery of EV-based COL1A1 mRNA may make for an effective protein-replacement therapy for the treatment of photoaged skin.
 You, Y., Tian, Y., Yang, Z., Shi, J., Kwak, K. J., Tong, Y., … & Lee, A. S. (2023). Intradermally delivered mRNA-encapsulating extracellular vesicles for collagen-replacement therapy. Nature Biomedical Engineering, 1-14.