Help us: Donate
Follow us on:
×

Menu

Back

Journal Club April Topic and Call Details

Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing

Journal ClubJournal Club
 

The Journal Club, hosted by Dr. Oliver Medvedik, will return at noon EST on Tuesday, April 27th. This month, we will be taking a look at a new paper and technique for the gene editing tool CRISPR/Cas9. The technique allows researchers to turn epigenetic methylation off and back on again, thus allowing the silencing and unsilencing of certain genes. This could be valuable in combating age-related diseases and reversing the harmful changes in gene expression that accompany aging.

A general approach for heritably altering gene expression has the potential to enable many discovery and therapeutic efforts. Here, we present CRISPRoff—a programmable epigenetic memory writer consisting of a single dead Cas9 fusion protein that establishes DNA methylation and repressive histone modifications. Transient CRISPRoff expression initiates highly specific DNA methylation and gene repression that is maintained through cell division and differentiation of stem cells to neurons. Pairing CRISPRoff with genome-wide screens and analysis of chromatin marks establishes rules for heritable gene silencing. We identify single guide RNAs (sgRNAs) capable of silencing the large majority of genes including those lacking canonical CpG islands (CGIs) and reveal a wide targeting window extending beyond annotated CGIs. The broad ability of CRISPRoff to initiate heritable gene silencing even outside of CGIs expands the canonical model of methylation-based silencing and enables diverse applications including genome-wide screens, multiplexed cell engineering, enhancer silencing, and mechanistic exploration of epigenetic inheritance.

As a Lifespan Hero, you can join us live on the call using the details below:

Topic: Journal Club
Time: Apr 27, 2021 12:00 PM Eastern Time (US and Canada)

Join Zoom Meeting
https://lifespan-io.zoom.us/j/81752321818?pwd=R0k2V0NIcVhpVHpOYUVzbWRXd0M2Zz09

Meeting ID: 817 5232 1818
Passcode: 210207
One tap mobile
+13126266799,,81752321818#,,,,*210207# US (Chicago)
+13462487799,,81752321818#,,,,*210207# US (Houston)

Dial by your location
+1 312 626 6799 US (Chicago)
+1 346 248 7799 US (Houston)
+1 646 876 9923 US (New York)
+1 669 900 6833 US (San Jose)
+1 253 215 8782 US (Tacoma)
+1 301 715 8592 US (Washington DC)
Meeting ID: 817 5232 1818
Passcode: 210207
Find your local number: https://lifespan-io.zoom.us/u/kcyhLN2OoG

Literature

Nuñez, J. K., Chen, J., Pommier, G. C., Cogan, J. Z., Replogle, J. M., Adriaens, C., … & Weissman, J. S. (2021). Genome-wide programmable transcriptional memory by CRISPR-based epigenome editing. Cell.

CategoryHeroes Corner
About the author

Steve Hill

Steve serves on the LEAF Board of Directors and is the Editor in Chief, coordinating the daily news articles and social media content of the organization. He is an active journalist in the aging research and biotechnology field and has to date written over 600 articles on the topic, interviewed over 100 of the leading researchers in the field, hosted livestream events focused on aging, as well as attending various medical industry conferences. His work has been featured in H+ magazine, Psychology Today, Singularity Weblog, Standpoint Magazine, Swiss Monthly, Keep me Prime, and New Economy Magazine. Steve is one of three recipients of the 2020 H+ Innovator Award and shares this honour with Mirko Ranieri – Google AR and Dinorah Delfin – Immortalists Magazine. The H+ Innovator Award looks into our community and acknowledges ideas and projects that encourage social change, achieve scientific accomplishments, technological advances, philosophical and intellectual visions, author unique narratives, build fascinating artistic ventures, and develop products that bridge gaps and help us to achieve transhumanist goals. Steve has a background in project management and administration which has helped him to build a united team for effective fundraising and content creation, while his additional knowledge of biology and statistical data analysis allows him to carefully assess and coordinate the scientific groups involved in the project.
No Comments
Write a comment:

*

Your email address will not be published.

This site uses Akismet to reduce spam. Learn how your comment data is processed.